Interested in making your own gas vesicles for ultrasound or MRI? An (excruciatingly) detailed protocol on GV production, purification, functionalization and imaging has just been published in Nature Protocols. Thanks to Anupama, George, Arash, Suchita, colleagues and collaborators on putting together this valuable resource.
Lakshmanan A#, Lu GJ#, Farhadi A#, Nety SP#, Kunth M, Lee-Gosselin A, Maresca D, Bourdeau RW, Yin M, Yan J, Witte C, Malounda D, Foster FS, Schröder L, Shapiro MG*. Preparation of biogenic gas vesicle nanostructures for use as contrast agents for ultrasound and MRI. Nature Protocols 12, 2050-2080 (2017). article | readcube
Dana Point, CA, August 26, 2017. Thanks to all the lab members, collaborators and friends who joined us!
Congratulations to David, Anu, Audrey, Johan, Yu-Li, Ray and our collaborator Dennis Kochmann on their article in Applied Physics Letters describing the development of nonlinear pulse sequences to maximize ultrasound contrast from gas vesicle protein nanostructures.
Article: Nonlinear ultrasound imaging of nanoscale acoustic biomolecules
See also a complementary article in Ultrasound in Medicine and Biology with collaborators at UofT detailing gas vesicles’ acoustic behavior.
Congratulations to Arnab, Di and Hunter on their article in Nature Communications describing the membrane water channel aquaporin as a new class of reporter genes for magnetic resonance imaging.
Article: Non-invasive imaging using reporter genes altering cellular water permeability
Press release: Visualizing Gene Expression with MRI
Congratulations to Dan, Mohamad, Brittany and Audrey on their article in Nature Chemical Biology describing the development of “bacterial thermostats” that allow microbial therapeutics to be controlled remotely using ultrasound, respond to fever, or know when they have exited their host.
Article: Tunable thermal bioswitches for in vivo control of microbial therapeutics.
Press release: Biologists Give Bacteria Thermostat Controls
Video: Scientists Engineer Therapeutic Bacteria to Listen to Us
Highlight: Nature Methods
Congratulations to Anupama, Arash, Suchita, Audrey, Ray and David on their cover article in the September issue of ACS Nano! Their work describes how gas vesicles can be engineered at the level of their protein composition and sequence to exhibit new acoustic properties and molecular targeting.
Article: Molecular Engineering of Acoustic Protein Nanostructures
Press release: Designing ultrasound tools with lego-like proteins
Hermosa Beach, California, July 16, 2016. Thanks to everyone who came!
Beautiful day at the ocean! Corona Del Mar, California, August 1, 2015
Our article on “Genetically encoded reporters for hyperpolarized xenon magnetic resonance imaging” has been published in Nature Chemistry. It describes how the the xenon-binding capabilities of gas vesicles allow them to be detected non-invasively at picomolar concentrations.
Link to the Article
Editor’s Choice in Science Translational Medicine
Thanks to all the lab members, family and friends who joined us for our first annual beach party at Bosla Chica beach!