{"id":1557,"date":"2023-01-02T10:20:16","date_gmt":"2023-01-02T18:20:16","guid":{"rendered":"https:\/\/shapirolab.caltech.edu\/?p=1557"},"modified":"2023-07-14T21:48:53","modified_gmt":"2023-07-15T04:48:53","slug":"acoustic-reporter-genes-2-0","status":"publish","type":"post","link":"https:\/\/shapirolab.caltech.edu\/?p=1557","title":{"rendered":"Acoustic Reporter Genes 2.0"},"content":{"rendered":"<div class=\"wp-block-image\">\n<figure class=\"alignright size-full is-resized\"><a href=\"https:\/\/shapirolab.caltech.edu\/wp-content\/uploads\/2023\/07\/Hurt-Buss-Duan-NBT-cover.jpg\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/shapirolab.caltech.edu\/wp-content\/uploads\/2023\/07\/Hurt-Buss-Duan-NBT-cover.jpg\" alt=\"\" class=\"wp-image-1558\" width=\"128\" height=\"168\"\/><\/a><\/figure>\n<\/div>\n\n\n<p>The first generation of acoustic reporter genes provided a key proof of concept but were insensitive, burdensome and impossible to image continuously. Welcome ARG 2.0! These new reporter genes provide much greater sensitivity, nonlinear ultrasound contrast, and ease-of-use for expression in a variety of cell types. Congrats to Rob, Marjorie, Tom and colleagues on this major advance! Constructs for mammalian cells and bacteria available on <a href=\"https:\/\/www.addgene.org\/browse\/article\/28229434\/\">Addgene<\/a>.\n\n<p>Hurt RC#, Buss MT#, Duan M#, Wong K, You MY, Sawyer DP, Swift MB, Dutka P, Barturen-Larrea P, Mittelstein DR, Jin Z, Abedi MH, Farhadi A, Dephande R, Shapiro MG*. <strong>Genomically mined acoustic reporter genes for real-time in vivo monitoring of tumors and tumor-homing bacteria.<\/strong>  <em>Nature Biotechnology<\/em> 41, 919 (2023). <a href=\"https:\/\/www.nature.com\/articles\/s41587-022-01581-y\">article<\/a><\/p>\n\n","protected":false},"excerpt":{"rendered":"<p>The first generation of acoustic reporter genes provided a key proof of concept but were insensitive, burdensome and impossible to image continuously. Welcome ARG 2.0! These new reporter genes provide &hellip;<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-1557","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/shapirolab.caltech.edu\/index.php?rest_route=\/wp\/v2\/posts\/1557","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/shapirolab.caltech.edu\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/shapirolab.caltech.edu\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/shapirolab.caltech.edu\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/shapirolab.caltech.edu\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1557"}],"version-history":[{"count":11,"href":"https:\/\/shapirolab.caltech.edu\/index.php?rest_route=\/wp\/v2\/posts\/1557\/revisions"}],"predecessor-version":[{"id":1645,"href":"https:\/\/shapirolab.caltech.edu\/index.php?rest_route=\/wp\/v2\/posts\/1557\/revisions\/1645"}],"wp:attachment":[{"href":"https:\/\/shapirolab.caltech.edu\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1557"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/shapirolab.caltech.edu\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1557"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/shapirolab.caltech.edu\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1557"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}